Neurotoxin Gene Profiling of Clostridium botulinum Types C and D Native to Different Countries within Europe
نویسندگان
چکیده
منابع مشابه
Production and Evaluation of a Recombinant Chimeric Vaccine against Clostridium botulinum Neurotoxin Types C and D
Bovine botulism is a fatal disease that is caused by botulinum neurotoxins (BoNTs) produced by Clostridium botulinum serotypes C and D and that causes great economic losses, with nearly 100% lethality during outbreaks. It has also been considered a potential source of human food-borne illness in many countries. Vaccination has been reported to be the most effective way to control bovine botulis...
متن کاملImmunogenic and Protective Potentials of Recombinant Receptor Binding Domain and a C-Terminal Fragment of Clostridium botulinum Neurotoxin Type E
Clostridium Botulinum Type E neurotoxin heavy chain consists of two domains: the translocation domain asthe N-terminal half and the binding domain as the Cterminal half (Hc). One effective way to neutralize botulinum neurotoxin is to inhibit binding of this toxin to neuromuscular synapses with antibodies against binding domain. Two synthetic genes, coding for Hc (the full length binding d...
متن کاملProduction and purification of Clostridium botulinum type C and D neurotoxin.
Neurotoxins of Clostridium botulinum are needed in basic neurologic research, but as therapeutic agent for certain neuromuscular disorders like strabism as well. A method for the production and purification of botulinum neurotoxins C and D is reported using a two-step hollow-fiber cross flow filtration and a newly developed chromatographic purification procedure. Hollow-fiber filtration proved ...
متن کاملUse of ganglioside affinity filters to identify toxigenic strains of Clostridium botulinum types C and D.
Clostridium botulinum neurotoxin is synthesized by toxic clones grown anaerobically on ganglioside affinity filters. The toxin binds to the filters and is detected by reaction with 125I-immunoglobulin G from type-specific antitoxin. Toxin spots from culture filtrates were similarly identified. The C. botulinum type C and D strains were selected for developing this affinity filter assay because ...
متن کاملImmunoprecipitation of native botulinum neurotoxin complexes from Clostridium botulinum subtype A strains.
Botulinum neurotoxins (BoNTs) naturally exist as components of protein complexes containing nontoxic proteins. The nontoxic proteins impart stability of BoNTs in the gastrointestinal tract and during purification and handling. The two primary neurotoxin complexes (TCs) are (i) TC1, consisting of BoNT, nontoxin-nonhemagglutinin (NTNH), and hemagglutinins (HAs), and (ii) TC2, consisting of BoNT a...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2012
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.07568-11